TAKAITACCE DA BAYANIN GWAJI
Kwayoyin cuta na Dengue, dangi na nau'ikan ƙwayoyin cuta daban-daban guda huɗu (Den 1,2,3,4), ƙwayoyin cuta na RNA marasa ƙarfi ne, lulluɓe, tabbataccen hankali.Kwayoyin cutar suna yaduwa ta hanyar sauro na dangin Stegemia masu ci da rana, musamman Aedes aegypti, da Aedes albopictus.A yau, fiye da mutane biliyan 2.5 da ke zaune a yankuna masu zafi na Asiya, Afirka, Ostiraliya, da Amurka suna cikin haɗarin kamuwa da cutar dengue.Kimanin mutane miliyan 100 na zazzabin dengue da kuma 250,000 na cutar zazzabin dengue mai barazana ga rayuwa suna faruwa kowace shekara a duk duniya1-3.
Gano serological na rigakafin IgM shine mafi yawan hanyar da aka fi sani don gano kamuwa da cutar dengue.Kwanan nan, gano antigens da aka saki yayin da ake yin kwafin ƙwayoyin cuta a cikin majinyacin da ya kamu da cutar ya nuna sakamako mai ban sha'awa.Yana ba da damar ganewar asali daga rana ta farko bayan fara zazzabi har zuwa ranar 9, da zarar lokacin asibiti na cutar ya ƙare, don haka yana ba da damar jiyya da wuri da wuri4-. An haɓaka gwajin Dengue IgG/IgM da sauri don gano antigen dengue da ke yawo a cikin jini, plasma ko duka jini.Za a iya yin gwajin ta ƙwararrun ma'aikatan da ba su horar da su ba ko kuma ƙwararrun ma'aikata, ba tare da kayan aikin dakin gwaje-gwaje ba.
KA'IDA
Gwajin gaggawa na Dengue IgG/IgM shine gwajin rigakafi na chromatographic na gefe.Kaset ɗin gwajin ya ƙunshi: 1) wani kushin conjugate mai launin burgundy mai ɗauke da dengue recombinant envelope antigens conjugated da colloid zinariya (dengue conjugates) da zomo IgG-gold conjugates,2) wani nitrocellulose membrane tsiri dauke da biyu gwajin makada (G da M bands) da kuma iko band (C band).An riga an yi amfani da rukunin G tare da maganin rigakafi don gano IgG anti-dengue virus, M band an lullube shi da antibody don gano IgM anti-dengue virus, kuma C band an riga an rufe shi da goat anti-dengue IgG.
Lokacin da aka ba da isasshen adadin samfurin gwaji a cikin rijiyar samfurin kaset ɗin, samfurin yana ƙaura ta hanyar aikin capillary a cikin kaset ɗin.IgG anti-dengue virus idan akwai a cikin samfurin zai ɗaure zuwa dengue conjugates.An kama immunocomplex ta hanyar reagent mai lullube akan rukunin G, yana samar da rukunin G mai launin burgundy, yana nuna sakamakon gwajin IgG na dengue kuma yana ba da shawarar kamuwa da cuta kwanan nan ko maimaitawa.IgM anti-dengue virus, idan akwai a cikin samfurin, zai ɗaure zuwa dengue conjugates.Immunocomplex an kama shi ta hanyar reagent wanda aka riga aka rufa akan M band, yana samar da band mai launin burgundy, yana nuna kwayar cutar dengue IgM tabbatacce sakamakon gwajin kuma yana ba da shawarar sabon kamuwa da cuta.
Rashin kowane nau'i na gwaji (G da M) yana nuna sakamako mara kyau. Gwajin ya ƙunshi iko na ciki (C band) wanda ya kamata ya nuna nau'in launi na burgundy na immunocomplex na goat anti rabbit IgG / rabbit IgG-gold conjugate ba tare da la'akari da ci gaban launi akan kowane nau'in T ba.In ba haka ba, sakamakon gwajin ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.
