TAKAITACCE DA BAYANIN GWAJI
Kwayoyin cuta na Dengue, dangi na nau'ikan ƙwayoyin cuta daban-daban guda huɗu (Den 1,2,3,4), ƙwayoyin cuta na RNA marasa ƙarfi ne, lulluɓe, tabbataccen hankali.Kwayoyin cutar suna yaduwa ta hanyar sauro na dangin Stegemia masu ci da rana, musamman Aedes aegypti, da Aedes albopictus.A yau, fiye da mutane biliyan 2.5 da ke zaune a yankuna masu zafi na Asiya, Afirka, Ostiraliya, da Amurka suna cikin haɗarin kamuwa da cutar dengue.Kimanin mutane miliyan 100 na zazzabin dengue da kuma 250,000 na cutar zazzabin dengue mai barazana ga rayuwa suna faruwa kowace shekara a duk duniya.
Gano serological na rigakafin IgM shine mafi yawan hanyar da aka fi sani don gano kamuwa da cutar dengue.Kwanan nan, gano antigens da aka saki yayin da ake yin kwafin ƙwayoyin cuta a cikin majinyacin da ya kamu da cutar ya nuna sakamako mai ban sha'awa.Yana ba da damar ganewar asali daga rana ta farko bayan fara zazzabi har zuwa ranar 9, da zarar lokacin asibiti na cutar ya ƙare, don haka yana ba da damar jiyya da wuri a sanya shi da sauri4-. An haɓaka gwajin Dengue NS1 da sauri don gano antigen dengue da ke yawo a cikin jini. , Plasma ko duka jini.Za a iya yin gwajin ta ƙwararrun ma'aikatan da ba su horar da su ba ko kuma ƙwararrun ma'aikata, ba tare da kayan aikin dakin gwaje-gwaje ba.
KA'IDA
Gwajin gaggawa na Dengue NS1 shine gwajin rigakafi na chromatographic na gefe.Kaset ɗin gwajin ya ƙunshi: 1) wani kushin conjugate mai launin burgundy mai ɗauke da linzamin kwamfuta anti-dengue NS1 antigen conjugated da colloid zinariya (Dengue Ab conjugates), 2) wani nitrocellulose membrane tsiri dauke da gwajin band (T band) da kuma kula da band (C). band).An riga an lulluɓe ƙungiyar T tare da antigen anti-dengue NS1, da kuma rukunin C
an riga an yi masa rufi da goat anti-mouse IgG antibody.Kwayoyin rigakafi don dengue antigen suna gane antigens daga dukkanin serotypes hudu na kwayar dengue.
Lokacin da aka ba da isasshen adadin samfurin gwaji a cikin rijiyar samfurin kaset, samfurin yana ƙaura ta hanyar aikin capillary a cikin kaset ɗin gwajin.Dengue NS1 Ag idan akwai a cikin samfurin zai ɗaure ga Dengue Ab conjugates.Ana kama immunocomplex akan membrane ta hanyar riga-kafin antiNS1 na linzamin kwamfuta, wanda ke samar da rukunin T mai launin burgundy, yana nuna sakamako mai kyau na Dengue Ag.
Rashin rukunin T yana nuna sakamako mara kyau.Gwajin ya ƙunshi iko na ciki (C band) wanda ya kamata ya nuna band mai launin burgundy na immunocomplex na goat anti-mouse IgG/ linzamin kwamfuta IgG-gold conjugate ko da kuwa kasancewar T band mai launi.In ba haka ba, sakamakon gwajin ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.
