TAKAITACCE DA BAYANIN GWAJI
Kwayar cutar Zika (Zika): galibi ana kamuwa da ita ta hanyar cizon sauro na Aedes, uwa da yaro, ƙarin jini da watsa jima'i.Saboda babu maganin alurar riga kafi a halin yanzu, mutane gabaɗaya suna kamuwa da kamuwa da cuta.Ana samar da rigakafin IgG/IgM mako guda bayan farawa, don haka gano IgG/IgM yana da mahimmanci ga farkon.
gano cutar Zika.Ana gano cutar ta Zika bisa binciken serological da keɓewar ƙwayar cuta a cikin beraye ko al'adun nama.IgM immunoassay ita ce hanya mafi amfani da gwajin gwaji.Gwajin gaggawa na zika IgM/IgG yana amfani da antigens na sake haduwa da aka samo daga sunadarin tsarin sa, yana gano IgM/IgG anti-zika a cikin jini ko plasma cikin mintuna 15.Za a iya yin gwajin ta ƙwararrun ma'aikatan da ba su da horo ko kuma ƙwararrun ma'aikata, ba tare da ƙaƙƙarfan kayan aikin dakin gwaje-gwaje ba.
KA'IDA
Gwajin gaggawa na Zika IgM/IgG shine gwajin rigakafi na chromatographic na gefe.Kaset ɗin gwajin ya ƙunshi: 1) wani kushin conjugate mai launin burgundy mai ɗauke da recombinant antigen da aka haɗa da colloid zinariya (Zika conjugates) da zomo IgG-gold conjugates, 2) wani nitrocellulose membrane tsiri dauke da gwajin gwaji guda biyu (M da G bands) da kuma sarrafawa. band (C band).An riga an riga an yi amfani da rukunin M band tare da monoclonal anti-human IgM don gano IgM anti-Zika, G band an riga an rufe shi da reagents don gano IgG anti-Zika, kuma rukunin C an riga an riga an rufe shi da rigakafin goat. zomo IgG.
Lokacin da aka ba da isasshen adadin samfurin gwaji a cikin rijiyar samfurin kaset ɗin, samfurin yana ƙaura ta hanyar aikin capillary a cikin kaset ɗin.Anti-Zika IgM idan akwai a cikin samfurin zai ɗaure ga masu haɗin Zika.An kama immunocomplex a jikin membrane ta riga-kafin rigakafin IgM na ɗan adam wanda aka riga aka rufa, yana samar da band ɗin M mai launin burgundy, yana nuna sakamako mai kyau na Zika IgM.
Anti-Zika IgG idan akwai a cikin samfurin zai ɗaure ga masu haɗin Zika.An kama immunocomplex ta hanyar reagents da aka riga aka rufa a jikin membrane, suna samar da rukunin G mai launin burgundy, yana nuna kyakkyawan sakamakon gwajin Zika IgG.Rashin kowane makada na gwaji (M da G) yana nuna sakamako mara kyau.Gwajin yana ƙunshe da iko na ciki (C band) wanda yakamata ya nuna band mai launin burgundy na immunocomplex na goat anti rabbit IgG/ rabbit IgG-gold conjugate ba tare da la’akari da ci gaban launi akan kowane rukunin gwajin ba.In ba haka ba, sakamakon gwajin ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.
