RV IgG Gwajin Saurin Gwajin da ba a yanke ba

RV IgG Gwajin Sauri da Ba a yanke Ba da Hoton da Aka Fitar

Gwajin gaggawa na RV IgG

Nau'in: Ba a yanke Sheet

Marka: Bio-mapper

Saukewa: RT0521

Misali: WB/S/P

Hankali: 91.10%

Musamman: 99%

Rubella galibi yana faruwa a cikin yara da matasa masu zuwa makaranta, kuma sama da kashi 80% na yawan jama'a suna da ingancin wannan rigakafin cutar.Mata masu ciki sun kamu da kwayar cutar rubella kafin makonni 20 na ciki, kuma abin da ya faru na teratogenesis na tayin ya yi yawa.Manya da yara masu kamuwa da cutar rubella na iya haifar da rashes na fata.ELISA: Mai ɗaukar hoto wanda aka lulluɓe da ƙwayar cutar rubella da ba a kunna ba zai iya ɗaure tare da takamaiman antibody a cikin samfurin da aka gwada, kuma ana gano maganin da ya dace da enzyme mai lakabi anti immunoglobulin ɗan adam da substrate.

Aika mana imel

Cikakken Bayani

Tags samfurin

Cikakken bayanin

(1) Don tarin samfuri da gwajin gwaji, samfurin jini ɗaya ne kawai ake buƙatar tattara.Duk da haka, idan ya zama dole a yi la'akari da matsayin rigakafi na mutanen da suka kamu da kwayar cutar, ya zama dole a dauki samfurori daga wadanda ake zargi da cutar rubella a cikin kwanaki 3 bayan farawar kurji da kuma kwanaki 14 zuwa 21 masu zuwa don ganowa lokaci guda.
(2) Daidai da ELISA na gaba ɗaya, ƙara PBS 50 a cikin kowane rami na sarrafawa da samfurin μ l.Ci gaba da ƙara samfurin 10 μl.Gasa a 25 ℃ na minti 45, wanke kuma bushe.
(3) Ƙara alamomin enzyme a kowace rijiya 250 μl.Ana amfani da wannan hanyar don adana zafi da wankewa.
(4) Ƙara pNPP substrate bayani 250 μl.Bayan adana zafi da wankewa ta hanyar guda ɗaya, ƙara 1mol / L sodium hydroxide 50 μ L Dakatar da amsawa, auna ƙimar ƙimar kowane rami a 405nm, kuma yanke hukunci sakamakon samfurin da aka gwada.
(5) Idan sakamako ne mai kyau, ana iya ƙara ƙarar samfurin don ƙayyade titer antibody, kwatanta sakamakon samfurori guda biyu a jere, kuma a yi hukunci.