TAKAITACCE DA BAYANIN GWAJI
Cutar zazzabin cizon sauro cuta ce da sauro ke haifarwa, ciwon jini, zazzabi da ke kamuwa da mutane sama da miliyan 200 kuma tana kashe mutane sama da miliyan 1 a kowace shekara.Yana haifar da nau'in Plasmodium guda huɗu: P. falciparum, P. vivax, P. ovale, da P. malariae.Wadannan plasmodia duk suna cutar da lalata erythrocytes na ɗan adam, suna haifar da sanyi, zazzabi, anemia, da splenomegaly.P. falciparum yana haifar da cututtuka fiye da sauran nau'in plasmodial kuma yana haifar da yawancin mutuwar zazzabin cizon sauro.P. falciparum da P. vivax sune cututtukan da aka fi sani da su, duk da haka, akwai bambance-bambancen yanki a cikin rarraba jinsuna.
A al'adance, ana gano cutar zazzabin cizon sauro ta hanyar bayyanar da kwayoyin halitta akan Giemsa masu kaurin smears na jini na gefe, kuma nau'in plasmodium daban-daban ana bambanta su ta hanyar bayyanar su a cikin erythrocytes masu kamuwa da cuta.Hanyar tana iya zama ingantacciyar ganewar asali, amma lokacin da aka yi amfani da su ne ta hanyar ƙwararrun microscols, wanda ke gabatar da manyan matsaloli don ƙasashe masu nisa na duniya.
An haɓaka gwajin gaggawa na zazzabin cizon sauro Pf/Pv Ag don magance waɗannan matsalolin.Yana amfani da ƙwayoyin rigakafi na musamman ga P. falciparum Histidine Rich Protein-II (pHRP-II) da zuwa P. vivax Lactate Dehydrogenase (Pv-LDH) don gano lokaci guda da bambanta kamuwa da cuta tare da P. falciparum da P. vivax.Za a iya yin gwajin ta ƙwararrun ma'aikatan da ba su horar da su ba ko kuma ƙwararrun ma'aikata, ba tare da kayan aikin dakin gwaje-gwaje ba
KA'IDA
Gwajin gaggawa na zazzabin cizon sauro Pf/Pv Ag shine gwajin rigakafi na chromatographic na gefe.Abubuwan gwajin tsiri sun ƙunshi: 1) kushin haɗin gwal mai launin burgundy mai ɗauke da linzamin kwamfuta anti-Pv-LDH antibody conjugated da colloid zinariya (Pv-LDH-gold conjugates) da linzamin kwamfuta anti-pHRP-II antibody conjugated tare da colloid zinariya (pHRP-II-zinariya conjugates) 2 da kuma bandeji bandeji (PHRP-II-zinariya conjugates), 2. band (C band).Pv band an riga an rufe shi da wani linzamin kwamfuta anti-Pv-LDH takamaiman antibody don gano kamuwa da cutar Pv, Pf band an riga an riga an rufe shi da polyclonal anti-pHRP-II antibodies don gano kamuwa da cutar Pf, kuma C band an rufe shi da goat anti-mouse IgG.
A lokacin tantancewar, an ba da isasshen adadin samfurin jini a cikin rijiyar samfurin (S) na kaset ɗin gwaji, ana ƙara buffer lysis zuwa rijiyar buffer (B).Buffer ɗin ya ƙunshi abin wanke-wanke wanda ke lalata ƙwayoyin jajayen jini kuma yana fitar da antigens iri-iri, waɗanda ke ƙaura ta hanyar aikin capillary a cikin tsiri da ke cikin kaset.Pv-LDH idan gabatarwa a cikin samfurin zai ɗaure ga haɗin gwal na Pv-LDH.An kama immunocomplex akan membrane ta hanyar riga-kafin anti-Pv-LDH mai rufi, yana samar da rukunin Pv mai launin burgundy, yana nuna sakamakon gwajin Pv mai kyau.A madadin, pHRP-II idan gabatarwa a cikin samfurin zai ɗaure zuwa pHRP-II-gold conjugates.An kama immunocomplex a kan membrane ta hanyar rigakafin anti-pHRP-II da aka riga aka rufe, suna samar da rukunin Pf mai launin burgundy, yana nuna sakamakon gwajin Pf mai kyau.Rashin kowane maƙallan gwaji yana nuna sakamako mara kyau.Gwajin ya ƙunshi iko na ciki (C band) wanda yakamata ya nuna ƙungiyar burgundy mai launi na immunocomplex na goat anti- linzamin kwamfuta IgG / linzamin kwamfuta IgG (anti-Pv-LDH da anti-pHRPII) conjugates na zinariya ba tare da la'akari da ci gaban launi akan kowane ɗayan gwajin ba.In ba haka ba, sakamakon gwajin ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.
