Filariasis IgG/IgM Kit ɗin Gwajin Saurin (Colloidal Gold)

Filariasis na lymphatic da aka fi sani da Elephantiasis, wanda akasari W. bancrofti da B. malayi ke haifarwa, yana shafar kusan mutane miliyan 120 sama da ƙasashe 80.Ana kamuwa da cutar ga mutane ta hanyar cizon sauro masu kamuwa da cuta wanda microflariae da aka sha daga wani mutum mai kamuwa da cuta ya tashi zuwa tsutsa mataki na uku.Gabaɗaya, ana buƙatar maimaitawa da tsawaita bayyanar da tsutsa masu kamuwa da cuta don kafa kamuwa da cutar ɗan adam.

Mahimmin ganewar asali na parasitologic shine nunin microflariae a cikin samfuran jini.Koyaya, wannan gwajin ma'auni na gwal an iyakance shi ta hanyar buƙatun tattara jinin dare da rashin isasshen hankali.Gano antigens masu yawo yana samuwa a kasuwa.Amfaninsa yana iyakance ga W. bancrofti.Bugu da ƙari, microfilaremia da antigenemia suna tasowa daga watanni zuwa shekaru bayan bayyanar.

Gano maganin rigakafi yana ba da hanya ta farko don gano kamuwa da cuta na filarial parasite.Kasancewar IgM zuwa antigens na parasites yana nuna kamuwa da cuta na yanzu, yayin da, IgG yayi daidai da ƙarshen matakin kamuwa da cuta ko kamuwa da cuta da ya gabata.Bugu da ƙari, gano antigens da aka adana yana ba da damar gwajin'pan-filaria' don yin aiki.Yin amfani da sunadaran sake haɗewa yana kawar da haɗin kai tare da mutanen da ke da wasu cututtukan parasitic.

Gwajin gaggawa na Filariasis IgG/IgM Combo yana amfani da recombinant da aka adana

antigens don gano IgG da IgM lokaci guda zuwa W. bancrofti da B. malayi parasites ba tare da ƙuntatawa akan tarin samfurori ba.

Filariasis IgG/IgM Kit ɗin Gwajin Saurin Gwajin Immunoassay na chromatographic na gefe.Cassette na gwajin ya ƙunshi: 1) wani kushin conjugate mai launin burgundy mai ɗauke da recombinant W. bancrofti da B. malayi antigens na gama-gari waɗanda aka haɗa tare da zinari na colloid (Filariasis conjugates) da zomo IgG-gold conjugates, 2) wani nitrocellulose membrane tsiri dauke da bandeji gwaji guda biyu (M da G band).M band an riga an rufe shi da monoclonal anti-human IgM don gano IgM anti-W. bancrofti da B. malayi, G band an riga an rufe shi da reagents don gano IgG anti-W.Bancrofti da B. malayi, kuma an riga an riga an riga an riga an rufe ƙungiyar C band da goat anti zomo IgG.

Lokacin da aka ba da isasshen adadin samfurin gwaji a cikin rijiyar samfurin kaset, samfurin yana ƙaura ta hanyar aikin capillary a cikin kaset ɗin.W. bancrofti ko B. malay IgM antibodies idan akwai a cikin samfurin za su ɗaure da Filariasis conjugates.Ana kama immunocomplex akan membrane ta hanyar riga-kafin IgM anti-an adam antibody, yana samar da bandeji mai launin burgundy, yana nuna sakamakon gwajin tabbatacce na W. bancrofti ko B. malayi IgM.

W. bancrofti ko B. malay IgG rigakafi idan akwai a cikin samfurin za su ɗaure da Filariasis conjugates.An kama immunocomplex da pre-rufin reagents a kan membrane, forming wani burgundy launi G band, nuna wani W. bancrofti ko B. malayi IgG tabbatacce sakamakon gwajin.

Rashin kowane makada na gwaji (M da G) yana nuna sakamako mara kyau.Gwajin yana ƙunshe da iko na ciki (C band) wanda yakamata ya nuna band mai launin burgundy na immunocomplex na goat anti rabbit IgG/ rabbit IgG-gold conjugate ba tare da la’akari da ci gaban launi akan kowane rukunin gwajin ba.In ba haka ba, sakamakon gwajin ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.