TAKAITACCE DA BAYANIN GWAJI
S. typhi, kwayar cutar Gram-negative ce ke haifar da zazzabin taifot.A duk duniya kimanin mutane miliyan 17 da mutuwar 600,000 suna faruwa kowace shekara.Marasa lafiya da suka kamu da kwayar cutar HIV suna cikin haɗarin kamuwa da cutar ta S. typhi sosai.Shaida na kamuwa da cutar H. pylori kuma yana nuna haɗarin kamuwa da zazzabin typhoid.1-5% na marasa lafiya sun zama mai ɗaukar nauyi mai ɗauke da S. typhi a cikin gallbladder.
Binciken asibiti na zazzabin typhoid ya dogara ne akan keɓewar S. typhi daga jini, bargon kashi ko wani takamaiman rauni na jiki.A cikin wuraren da ba za su iya yin wannan aiki mai rikitarwa da cin lokaci ba, ana amfani da gwajin Filix-Widal don sauƙaƙe ganewar asali.Koyaya, iyakoki da yawa suna haifar da matsaloli a cikin fassarar gwajin Widal.
Sabanin haka, Kit ɗin gwajin gaggawa na Typhoid IgG/IgM gwaji ne mai sauƙi kuma mai sauri.Gwajin a lokaci guda yana ganowa kuma ya bambanta IgG da IgM antibodies zuwa S. typhi takamaiman antigen a cikin dukkanin samfuran jini don haka yana taimakawa wajen tantance halin yanzu ko na baya ga S. typhi.
KA'IDA
Gwajin gaggawa na Typhoid IgG/IgM Combo Rapid Test shine chromatographic kwarara na gefe
immunoassay.Kaset ɗin gwajin ya ƙunshi: 1) kushin conjugate mai launin burgundy mai ɗauke da recombinant S. typhoid H antigen da O antigen conjugated da colloid zinariya (Typhoid conjugates) da zomo IgG-gold conjugates, 2) wani nitrocellulose membrane tsiri dauke da biyu gwajin makada (M da kuma G band).An riga an riga an riga an lulluɓe ƙungiyar M tare da IgM anti-mutum na monoclonal don gano IgM anti-S.typhi, G band an riga an rufe shi da reagents don gano IgG
anti-S.typhi, kuma rukunin C an riga an rufe shi da goat anti zomo IgG.
Lokacin da aka ba da isasshen adadin samfurin gwaji a cikin rijiyar samfurin kaset ɗin, samfurin yana ƙaura ta hanyar aikin capillary a cikin kaset ɗin.Anti-S.typhi IgM idan akwai a cikin samfurin za su daure da typhoid conjugates.Ana kama immunocomplex akan membrane ta hanyar riga-kafi na anti-an adam IgM antibody, yana samar da band M mai launin burgundy, yana nuna sakamakon gwajin S. typhi IgM mai kyau.
Anti-S.typhi IgG idan akwai a cikin samfurin za ta ɗaure da haɗin gwiwar Typhoid.Immunocomplex an kama shi ta hanyar reagents da aka riga aka rufawa akan membrane, suna samar da rukunin G mai launin burgundy, yana nuna kyakkyawan sakamakon gwajin S. typhi IgG.
Rashin kowane makada na gwaji (M da G) yana nuna sakamako mara kyau.Gwajin yana ƙunshe da iko na ciki (C band) wanda yakamata ya nuna band mai launin burgundy na immunocomplex na goat anti rabbit IgG/ rabbit IgG-gold conjugate ba tare da la’akari da ci gaban launi akan kowane rukunin gwajin ba.In ba haka ba, sakamakon gwajin ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.
