Cikakken bayanin
Hanyar dubawa
Akwai manyan hanyoyin bincike guda uku don toxoplasmosis: ganewar asali na pathogenic, ganewar rigakafi da ganewar kwayoyin halitta.Jarabawar cuta ta musamman ta haɗa da gano ilimin tarihi, rigakafin dabbobi da keɓewa, da al'adun tantanin halitta.Hanyoyin ƙwararrun ƙwayoyin cuta na gama gari sun haɗa da gwajin rini, gwajin hemagglutination na kaikaice, gwajin rigakafin rigakafi kaikaice na immunofluorescence da gwajin immunosorbent mai alaƙa da enzyme.Fahimtar ƙwayoyin ƙwayoyin cuta sun haɗa da fasahar PCR da fasahar haɓakar acid nucleic.
Gwajin jiki mai ciki na mata masu ciki ya haɗa da gwajin da ake kira TORCH.TORCH haɗe ne na harafin farko na sunan Ingilishi na ƙwayoyin cuta da yawa.Harafin T yana nufin Toxoplasma gondii.(Sauran haruffa suna wakiltar syphilis, cutar rubella, cytomegalovirus da cutar ta herpes simplex bi da bi.)
Duba ka'ida
Gwajin cuta
1. Kai tsaye microscopic jarrabawa na haƙuri ta jini, kasusuwa na kasusuwa ko cerebrospinal ruwa, pleural da ascites, sputum, bronchoalveolar lavage ruwa, mai ruwa jin daɗi, ruwa amniotic, da dai sauransu ga smears, ko Lymph nodes, tsokoki, hanta, placenta da sauran rayayyun nama sassan, ga Reich ko Jiing rate ne m, amma ga Reich ko Jiing rate ne m cystitis.Hakanan ana iya amfani dashi don immunofluorescence kai tsaye don gano Toxoplasma gondii a cikin kyallen takarda.
2. Cutar da dabbobi ko al'adar nama Ɗauki ruwan jiki ko dakatarwar nama don a gwada shi kuma a sa shi cikin kogon ciki na beraye.Kamuwa da cuta na iya faruwa kuma ana iya samun ƙwayoyin cuta.Lokacin da ƙarni na farko na inoculation ba shi da kyau, ya kamata a watsa shi a makanta har sau uku.Ko don al'adun nama (kodar biri ko ƙwayoyin koda na alade) don ware da gano Toxoplasma gondii.
3. Fasaha hybridization DNA Masana cikin gida sun yi amfani da 32P masu lakabin binciken da ke dauke da takamaiman jerin DNA na Toxoplasma gondii a karon farko don gudanar da haɓakar kwayoyin halitta tare da sel ko kyallen takarda DNA a cikin jini na marasa lafiya, kuma sun nuna cewa ƙayyadaddun makada ko tabo sun kasance halayen halayen kirki.Duk ƙayyadaddun ƙayyadaddun abubuwa da hankali sun kasance babba.Bugu da kari, an kuma kafa kwayar cutar ta polymerase (PCR) a kasar Sin don tantance cutar, kuma idan aka kwatanta da yadda ake yin gwajin kwayoyin cuta, da allurar rigakafin dabbobi da hanyoyin gwajin rigakafi, ya nuna cewa yana da matukar takamaimai, mai hankali da sauri.
Gwajin rigakafi
1. Antigens da ake amfani da su don gano maganin rigakafi sun haɗa da tachyzoite soluble antigen (cytoplasmic antigen) da kuma membrane antigen.Maganin rigakafi na tsohon ya bayyana a baya (an gano shi ta hanyar gwajin tabo da gwajin immunofluorescence na kai tsaye), yayin da na karshen ya bayyana daga baya (an gano shi ta gwajin hemagglutination kai tsaye, da sauransu).A lokaci guda, hanyoyin ganowa da yawa na iya taka rawa mai ma'amala da haɓaka ƙimar ganowa.Saboda Toxoplasma gondii na iya wanzuwa a cikin sel na ɗan adam na dogon lokaci, yana da wuya a iya bambanta kamuwa da cuta na yanzu ko kamuwa da cuta ta baya ta hanyar gano ƙwayoyin cuta.Ana iya yin hukunci bisa ga titer antibody da canje-canjen sa.
2. Ana amfani da maganin antigen don gano ƙwayoyin cuta (tachyzoites ko cysts) a cikin kwayoyin halitta, metabolites ko samfurori na lysis (antigens masu rarraba) a cikin jini da ruwan jiki ta hanyar hanyoyin rigakafi.Hanya ce ta dogara ga farkon ganewar asali da tabbataccen ganewar asali.Masana a gida da waje sun kafa McAb ELISA da sandwich ELISA tsakanin McAb da multiantibody don gano antigen da ke yaduwa a cikin maganin marasa lafiya masu tsanani, tare da hankali na 0.4 μ G / ml na antigen.
Abun ciki na Musamman
Tsarin Samar da Gwajin Saurin da ba a yanke ba
