Cikakken bayanin
Gano ƙwayar ƙwayar cuta ta tumaki ta ƙunshi microplate wanda aka riga aka rufa da shi tare da antigen protein pox na tumaki, alamomin enzyme da sauran abubuwan da ke tallafawa, kuma ana amfani da ka'idar immunoassay mai alaƙa da enzyme (ELISA) don gano ƙwayar cutar tumaki a cikin samfurin ƙwayar tumaki.A lokacin gwajin, ana saka maganin da aka sarrafa da samfurin da za a bincika a cikin farantin microplate, kuma idan samfurin ya ƙunshi ƙwayoyin cuta na tumaki bayan shiryawa, za a ɗaure shi da antigen akan farantin microplate, kuma sauran abubuwan da ba a ɗaure ba za a cire su bayan wankewa;Sannan ƙara alamar enzyme don ɗaure musamman ga hadadden antigen-antibody akan farantin microplate;An cire alamomin enzyme da ba a ɗaure ba ta hanyar wankewa, kuma an ƙara maganin TMB a cikin rijiyoyin, kuma samfurin blue ya kasance ta hanyar amsawar microplate conjugates, kuma zurfin launi yana da alaƙa da takamaiman adadin ƙwayoyin rigakafi da ke cikin samfurin.Bayan da aka ƙara maganin ƙarewa don kawo ƙarshen amsawa, samfurin ya juya rawaya;Ƙimar shayarwa a cikin kowane rijiyar da mai karatu ta microplate ta ƙayyade a tsawon 450 nm don sanin ko samfurin ya ƙunshi ƙwayoyin rigakafi na tumaki.