TAKAITACCE DA BAYANIN GWAJI
Cutar zazzabin cizon sauro cuta ce da sauro ke haifarwa, ciwon jini, zazzabi da ke kamuwa da mutane sama da miliyan 200 kuma tana kashe mutane sama da miliyan 1 a kowace shekara.Yana haifar da nau'in Plasmodium guda huɗu: P. falciparum, P. vivax, P. ovale, da P. malariae.Wadannan plasmodia duk suna cutar da lalata erythrocytes na ɗan adam, suna haifar da sanyi, zazzabi, anemia, da splenomegaly.P. falciparum yana haifar da cututtuka fiye da sauran nau'in plasmodial kuma yana haifar da yawancin mutuwar zazzabin cizon sauro.P. falciparum da P. vivax sune cututtukan da aka fi sani da su, duk da haka, akwai bambance-bambancen yanki a cikin rarraba jinsuna.
A al'adance, ana gano cutar zazzabin cizon sauro ta hanyar bayyanar da kwayoyin halitta akan Giemsa masu kaurin smears na jini na gefe, kuma nau'in plasmodium daban-daban ana bambanta su ta hanyar bayyanar su a cikin erythrocytes masu kamuwa da cuta1.Dabarar tana da ikon tabbatar da ingantacciyar ganewar asali kuma abin dogaro, amma sai a lokacin da ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun za ta yi amfani da ƙayyadaddun ƙayyadaddun ƙayyadaddun ka’idojin 2, wanda ke ba da babbar cikas ga wurare masu nisa da matalauta na duniya.
An samar da Kit ɗin gwajin gaggawa na zazzabin cizon sauro Pf / Pan Antigen don magance waɗannan matsalolin.Gwajin yana amfani da nau'i-nau'i na monoclonal da polyclonal zuwa P. falciparum takamaiman furotin, Histidine Repeat Protein II (pHRP-II), da kuma nau'i na kwayoyin halitta na monoclonal zuwa plasmodium Lactate Dehydrogenase (pLDH), furotin da aka samar da nau'in nau'i hudu na plasmodium, don haka yana ba da damar ganowa na lokaci guda tare da kowane nau'i na kamuwa da cuta ko wani nau'i na plasmodium. .Za a iya yin ta ta ƙwararrun ƙwararrun ƙwararrun ma'aikata, ba tare da kayan aikin dakin gwaje-gwaje ba.
KA'IDA
Pf/ Pan Malaria Kit ɗin Gwajin Saurin Gwajin ƙwayar cuta ce ta ƙwanƙwasa ta chromatographic immunoassay.Abubuwan gwajin gwajin sun ƙunshi: 1) pad mai launin burgundy mai ɗauke da linzamin kwamfuta anti-pHRP-II antibody conjugated da colloid zinariya (pHRP II-gold conjugates) da linzamin kwamfuta anti-pLDH antibody conjugated da colloid zinariya (pLDH-gold conjugates),
2) nitrocellulose membrane tsiri dauke da nau'ikan gwaji guda biyu (Pan da Pv bands) da kuma bandungiyar sarrafawa (C band).Pan band an riga an riga an rufe shi da monoclonal anti-pLDH antibody wanda za'a iya gano kamuwa da cuta tare da kowane nau'in nau'in plasmodia guda huɗu, Pf band an riga an rufe shi da polyclonal anti-pHRP-II antibodies don gano kamuwa da cutar Pf, kuma rukunin C yana mai rufi da goat anti-mouse IgG.
A lokacin tantancewar, an ba da isasshen adadin samfurin jini a cikin rijiyar samfurin (S) na kaset ɗin gwaji, ana ƙara buffer lysis zuwa rijiyar buffer (B).Makullin ya ƙunshi abin wanke-wanke wanda ke lalata ƙwayoyin jajayen jini kuma ya saki nau'ikan antigens na plasmodium, waɗanda ke ƙaura ta hanyar aikin capillary a cikin tsiri da ke cikin kaset.pHRP-II idan gabatarwa a cikin samfurin zai ɗaure zuwa pHRP II-gold conjugates.Ana kama immunocomplex akan membrane ta hanyar riga-kafi anti-pHRPII antibodies, samar da wani burgundy launi Pf band, yana nuna wani Pf tabbatacce sakamakon gwajin.pLDH idan aka gabatar a cikin samfurin zai ɗaure zuwa pLDH conjugates na zinariya.Ana kama immunocomplex akan membrane ta hanyar riga-kafin anti pLDH wanda aka riga aka rufa, yana samar da bandungiyar Pan mai launin burgundy, yana nuna sakamakon gwajin ingancin plasmodium.Idan babu Pan band, ana iya ba da shawarar sakamako mai kyau na kowane ɗayan ƙwayoyin plasmodia guda uku.
Rashin kowane maƙallan gwaji (Pan da Pf) yana nuna sakamako mara kyau.Gwajin ya ƙunshi iko na ciki (C band) wanda yakamata ya nuna ƙungiyar burgundy mai launi na immunocomplex na goat anti-mouse IgG / linzamin kwamfuta IgG (pHRP-II da pLDH-gold conjugates) ba tare da la'akari da haɓakar launi akan kowane ɗayan gwajin ba.In ba haka ba, sakamakon gwajin ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.
