TAKAITACCE DA BAYANIN GWAJI
Cutar zazzabin cizon sauro cuta ce da sauro ke haifarwa, ciwon jini, zazzabi da ke kamuwa da mutane sama da miliyan 200 kuma tana kashe mutane sama da miliyan 1 a kowace shekara.Yana haifar da nau'in Plasmodium guda huɗu: P. falciparum, P. vivax, P. ovale, da P. malariae.Wadannan plasmodia duk suna cutar da lalata erythrocytes na ɗan adam, suna haifar da sanyi, zazzabi, anemia, da splenomegaly.P. falciparum yana haifar da cututtuka fiye da sauran nau'in plasmodial kuma shine mafi yawan mutuwar zazzabin cizon sauro, kuma yana daya daga cikin cututtuka guda biyu da aka fi sani da malaria.
A al'adance, ana gano cutar zazzabin cizon sauro ta hanyar bayyanar da kwayoyin halitta akan Giemsa masu kaurin smears na jini na gefe, kuma nau'in plasmodium daban-daban ana bambanta su ta hanyar bayyanar su a cikin erythrocytes masu kamuwa da cuta.Hanyar tana iya zama ingantacciyar ganewar asali, amma lokacin da aka yi amfani da su ne ta hanyar ƙwararrun microscols, wanda ke gabatar da manyan matsaloli don ƙasashe masu nisa na duniya.
An haɓaka gwajin gaggawa na Pf Ag don magance waɗannan cikas.Yana gano Pf takamaiman antigen pHRP-II a cikin samfurin jinin ɗan adam.Za a iya yin ta ta ƙwararrun ƙwararrun ƙwararrun ma'aikata, ba tare da kayan aikin dakin gwaje-gwaje ba.
KA'IDA
Gwajin gaggawa na Pf Ag shine immunoassay na chromatographic na gefe.Abubuwan gwajin gwajin sun ƙunshi: 1) pad mai launin burgundy mai haɗakar da anti-pHRP-II antibody monoclonal conjugated da colloid zinariya (pHRP II-gold conjugates), 2) wani nitrocellulose membrane tsiri dauke da gwajin band (Pf) da kuma iko band (C band).Pf band an riga an rufe shi da polyclonal anti-pHRP-II antibodies, kuma rukunin C an riga an rufe shi da goat anti-mouse IgG.
A lokacin tantancewar, an ba da isasshen adadin samfurin jini a cikin rijiyar samfurin (S) na kaset ɗin gwaji, ana ƙara buffer lysis zuwa rijiyar buffer (B).Makullin yana ƙunshe da abin wanke-wanke wanda ke lalata ƙwayoyin jajayen jini kuma ya saki antigen pHRPII, wanda ke ƙaura ta hanyar aikin capillary a cikin tsiri da ke cikin kaset.pHRP-II idan gabatarwa a cikin samfurin zai ɗaure zuwa pHRP II-gold conjugates.Ana kama immunocomplex akan membrane ta hanyar riga-kafi polyclonal antipHRP II antibodies, samar da rukunin Pf mai launin burgundy, yana nuna sakamakon gwajin Pf mai kyau.
Rashin ƙungiyar Pf yana nuna sakamako mara kyau.Gwajin ya ƙunshi iko na ciki (C band) wanda yakamata ya nuna band mai launin burgundy na immunocomplex na goat anti-mouse IgG / linzamin kwamfuta IgG (pHRP II-gold conjugates) ba tare da la'akari da ci gaban launi akan kowane rukunin Pf ba.In ba haka ba, sakamakon gwajin ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.
