TAKAITACCE DA BAYANIN GWAJI
Filariasis na lymphatic da aka fi sani da Elephantiasis, wanda akasari W. bancrofti da B. malayi ke haifarwa, yana shafar kusan mutane miliyan 120 sama da ƙasashe 80.Ana kamuwa da cutar ga mutane ta hanyar cizon sauro masu kamuwa da cuta wanda microflariae da aka sha daga wani mutum mai kamuwa da cuta ya tashi zuwa tsutsa mataki na uku.Gabaɗaya, ana buƙatar maimaitawa da tsawaita bayyanar da tsutsa masu kamuwa da cuta don kafa kamuwa da cutar ɗan adam.
Mahimmin ganewar asali na parasitologic shine nunin microflariae a cikin samfuran jini.Koyaya, wannan gwajin ma'auni na gwal an iyakance shi ta hanyar buƙatun tattara jinin dare da rashin isasshen hankali.Gano antigens masu yawo yana samuwa a kasuwa.Amfaninsa yana iyakance ga W. bancrofti.Bugu da ƙari, microfilaremia da antigenemia suna tasowa daga watanni zuwa shekaru bayan bayyanar.
Gano maganin rigakafi yana ba da hanya ta farko don gano kamuwa da cuta na filarial parasite.Kasancewar IgM zuwa antigens na parasites yana nuna kamuwa da cuta na yanzu, yayin da, IgG yayi daidai da ƙarshen matakin kamuwa da cuta ko kamuwa da cuta da ya gabata.Bugu da ƙari, gano antigens da aka adana yana ba da damar gwajin 'pan-filaria' don aiki.Yin amfani da sunadaran sake haɗewa yana kawar da haɗin kai tare da mutanen da ke da wasu cututtukan parasitic.
Gwajin Filariasis Ab Mai Sauri yana amfani da antigens da aka adana don gano Antibody zuwa W. bancrofti da B. malayi parasites ba tare da ƙuntatawa akan tarin samfurin ba.
KA'IDA
Gwajin Filariasis Ab Rapid shine gwajin rigakafi na chromatographic na gefe.Kaset ɗin gwajin ya ƙunshi: 1) kushin conjugate mai launin burgundy mai ɗauke da recombinant Filariasis takamaiman antigen da aka haɗa tare da zinare na colloid (Filariasis conjugates) da zomo IgG-gold conjugates, 2) wani nitrocellulose membrane tsiri dauke da gwajin band (T band) da kuma sarrafawa. band (C band).An riga an riga an lulluɓe ƙungiyar T tare da antigen Filariasis wanda ba a haɗa shi ba, kuma rukunin C an riga an riga an yi masa rufi da rigar rigakafin zomo IgG.
Lokacin da aka ba da isasshen adadin samfurin gwaji a cikin rijiyar samfurin kaset ɗin, samfurin yana ƙaura ta hanyar aikin capillary a cikin kaset ɗin.AntiFilariasis Ab idan akwai a cikin samfurin zai ɗaure da Filariasis conjugates.Ana kama immunocomplex akan membrane ta hanyar antigen da aka riga aka rufawa, yana samar da rukunin T mai launin burgundy, yana nuna aFilariasis Ab tabbatacce sakamakon gwajin.Rashin rukunin T yana nuna sakamako mara kyau.Gwajin yana ƙunshe da iko na ciki (C band) wanda yakamata ya nuna band mai launin burgundy na immunocomplex na goat anti-zomo IgG/ rabbit IgG-gold conjugate ba tare da la’akari da ci gaban launi akan rukunin T ba.In ba haka ba, sakamakon gwajin ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.
