TAKAITACCE DA BAYANIN GWAJI
Chlamydia pneumoniae (C. pneumoniae) wani nau'in kwayoyin cuta ne na yau da kullun kuma babban dalilin ciwon huhu a duniya.Kusan kashi 50 cikin ɗari na manya suna da shaidar kamuwa da cuta a baya ta hanyar shekaru 20, kuma sake kamuwa da cuta daga baya a rayuwa ya zama ruwan dare.Yawancin karatu sun ba da shawarar haɗin kai tsaye tsakanin kamuwa da cutar ciwon huhu da sauran cututtuka masu kumburi irin su atherosclerosis, m exacerbations na COPD, da kuma asma.
Ganewar kamuwa da cutar ciwon huhu na C. yana da ƙalubale saboda saurin yanayin ƙwayoyin cuta, da yawa mai yawa, da yuwuwar jigilar asymptomatic na wucin gadi.Ƙididdiga hanyoyin gwaje-gwajen bincike sun haɗa da warewar kwayoyin halitta a cikin al'adun tantanin halitta, ƙididdigar serological da PCR.Microimmunofluorescence gwajin (MIF), shine "ma'auni na zinariya" na yanzu don ganewar asali na serological, amma ƙididdigar har yanzu ba ta da daidaituwa kuma yana da kalubale a fasaha.Immunoassays na rigakafi shine mafi yawan gwaje-gwajen serology da aka yi amfani da su kuma kamuwa da cutar chlamydial na farko yana da alaƙa da babban martanin IgM a cikin makonni 2 zuwa 4 da jinkirin amsawar IgG da IgA a cikin makonni 6 zuwa 8.Koyaya, a cikin sake haifuwa, matakan IgG da IgA suna tashi da sauri, sau da yawa a cikin makonni 1-2 yayin da matakan IgM na iya da wuya a gano su.Saboda wannan dalili, ƙwayoyin rigakafi na IgA sun nuna alamar abin dogara na rigakafi na farko, na yau da kullum da kuma cututtuka na yau da kullum musamman idan aka haɗu tare da gano IgM.
KA'IDA
Chlamydia pneumoniae IgG/IgM Rapid Test Kit yana dogara ne akan ka'idar gwaji mai inganci na immunochromatographic don tantance Chlamydia pneumoniae IgG/IgM antibody a cikin jinin mutum, plasma ko duka jini.StripA ya ƙunshi: 1) wani burgundy mai launi mai launi na Cc. pneumoniae Antigen conjugates), 2) wani nitrocellulose membrane tsiri dauke da gwajin band (T band) da kuma kula da band (C band).An riga an riga an lulluɓe ƙungiyar T tare da linzamin kwamfuta anti-human IgG antibody, kuma rukunin C an riga an yi masa rufi da goat anti-mouse IgG antibody.Strip B ya ƙunshi : 1) wani pad mai launin burgundy mai ƙunshe da C. pneumoniae antigen mai haɗaka da zinariya colloid (C. pneumoniae Antigen conjugates), 2) a
nitrocellulose membrane tsiri dauke da wani gwajin band (T band) da kuma kula da band (C band).An riga an riga an lulluɓe ƙungiyar T tare da linzamin kwamfuta anti-human IgM antibody, kuma rukunin C an riga an yi masa rufi da goat anti-mouse IgG antibody.
Strip A: Lokacin da aka ba da isasshen adadin gwajin gwaji a cikin rijiyar samfurin kaset ɗin, samfurin yana ƙaura ta hanyar aikin capillary a cikin kaset.C.pneumoniae IgG antibody idan akwai a cikin samfurin zai ɗaure ga C. pneumoniae Antigen conjugates.An kama immunocomplex akan membrane ta hanyar riga-kafin Mouse anti-human IgG antibody, yana samar da rukunin T mai launin burgundy,
yana nuna sakamakon gwajin ingancin C. pneumoniae IgG.Rashin rukunin T yana nuna sakamako mara kyau.Gwajin yana ƙunshe da iko na ciki (C band) wanda yakamata ya nuna band mai launin burgundy na immunocomplex na goat anti-mouse IgG/mouse IgGgold conjugate ba tare da la’akari da kasancewar rukunin T masu launi ba.In ba haka ba, sakamakon gwajin
ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.
Strip B: Lokacin da aka ba da isasshen adadin samfurin gwaji a cikin rijiyar samfurin kaset ɗin, samfurin yana ƙaura ta hanyar aikin capillary a cikin kaset.C.pneumoniae IgM antibody idan akwai a cikin samfurin zai ɗaure ga C. pneumoniae Antigen conjugates.Immunocomplex an kama shi a jikin membrane ta riga-kafin IgM na Mouse anti-human antibody, yana samar da rukunin T mai launin burgundy,
yana nuna sakamakon gwajin ingancin C. pneumoniae IgM.Rashin rukunin T yana nuna sakamako mara kyau.Gwajin yana ƙunshe da iko na ciki (C band) wanda yakamata ya nuna band mai launin burgundy na immunocomplex na goat anti-mouse IgG/mouse IgGgold conjugate ba tare da la’akari da kasancewar rukunin T masu launi ba.In ba haka ba, sakamakon gwajin ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.
