TAKAITACCE DA BAYANIN GWAJI
Zawo na daya daga cikin dalilan da ke haifar da cutukan yara da mace-mace a duniya, wanda ke haifar da mutuwar mutane miliyan 2.5 a duk shekara.Cutar ta Rotavirus ita ce kan gaba wajen haifar da zawo mai tsanani a jarirai da yara ‘yan kasa da shekaru biyar, wanda ya kai kashi 40% -60 na cutar gastroenteritis mai tsanani da kuma haddasa mutuwar yara kusan 500,000 a kowace shekara.Ya zuwa shekaru biyar, kusan kowane yaro a duniya ya kamu da cutar rotavirus aƙalla sau ɗaya.Tare da cututtuka na gaba, ana haifar da amsa mai girma, heterotypic antibody;don haka manya ba kasafai ake samun matsala ba.
Zuwa yau rukunoni bakwai na rotaviruses (kungiyoyin AG) sun keɓe kuma
halin.Rukunin A rotavirus, rotavirus da aka fi sani, yana haifar da fiye da kashi 90% na duk cututtukan Rotavirus a cikin mutane.Rotavirus ana ɗaukarsa da farko ta hanyar fecaloral, kai tsaye daga mutum zuwa mutum.Masu ciwon ƙwayar cuta a cikin stool sun kai matsakaicin jim kaɗan bayan fara rashin lafiya, sannan su ƙi.Lokacin shiryawa na kamuwa da cutar rotavirus yawanci kwana ɗaya ne zuwa uku kuma yana biye da ita gastroenteritis tare da matsakaicin tsawon kwanaki uku zuwa bakwai.Alamomin cutar sun hada da gudawa mai laushi, mai ruwa da ruwa zuwa gudawa mai tsanani tare da zazzabi da amai.
Ana iya gano kamuwa da kamuwa da cuta tare da rotavirus bayan gano cutar gastroenteritis a matsayin sanadin cutar gudawa mai tsanani a cikin yara.Kwanan nan, takamaiman ganewar asali na kamuwa da cuta tare da rotavirus ya zama samuwa ta hanyar gano antigen na ƙwayar cuta a cikin stool ta hanyoyin immunoassay kamar su latex agglutination assay, EIA, da kuma ƙwanƙwasa chromatographic immunoassay.
Gwajin Rotavirus Ag Rapid Gwajin gwaji ne na chromatographic immunoassay na gefe wanda ke amfani da takamaiman ƙwayoyin rigakafi guda biyu don gano antigen na rotavirus da kyau a cikin samfurin fecal.Ana iya yin gwajin ba tare da kayan aikin dakin gwaje-gwaje ba, kuma ana samun sakamakon a cikin mintuna 15.
KA'IDA
Gwajin Rotavirus Ag Rapid Gwajin gwajin gwaji ne na chromatographic na gefe.Tarin gwajin ya ƙunshi: 1) kushin conjugate mai launin burgundy mai ɗauke da kwayar cutar monoclonal anti-rotavirus antibody wanda aka haɗa tare da zinare na colloidal (anti-rotavirus conjugates) da kuma maganin rigakafi mai haɗawa da gwal colloidal, 2) membrane nitrocellulose mai ɗauke da layin gwaji (T) layi) da kuma layin sarrafawa (layin C).An riga an riga an yi rufin layin T tare da wani maganin rigakafin rotavirus na monoclonal, kuma an riga an riga an riga an riga an lulluɓe layin C tare da rigakafin layin sarrafawa.
Lokacin da isasshen adadin samfurin da aka fitar ya bazu cikin rijiyar samfurin kaset ɗin gwajin, samfurin yana ƙaura ta hanyar aikin capillary a cikin kaset ɗin.Rotavirus Ag, idan akwai a cikin samfurin, zai ɗaure da anti-rotavirus conjugates.An kama immunocomplex a kan membrane ta hanyar rigakafin rotavirus wanda aka rigaya ya kafa wani layin T mai launin burgundy, yana nuna sakamakon gwajin rotavirus. yana nuna mummunan sakamako na rotavirus.Gwajin ya ƙunshi kulawar ciki (layin C), wanda ya kamata ya nuna layin launi mai launi na burgundy na immunocomplex na ƙwayoyin rigakafi, ba tare da la'akari da ci gaban launi akan layin T ba.In ba haka ba, sakamakon gwajin ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.
