TAKAITACCE DA BAYANIN GWAJI
Helicobacter pylori yana hade da cututtuka iri-iri na gastrointestinal ciki har da dyspepsia marasa ciwon ciki, duodenal ulcers da ciki da kuma aiki, gastritis na kullum.Yawan kamuwa da cutar H. pylori zai iya wuce kashi 90 cikin 100 a cikin marasa lafiya da alamu da alamun cututtuka na ciki.Binciken da aka yi kwanan nan ya nuna ƙungiyar kamuwa da cutar H. pylori tare da ciwon daji na ciki.
Ana iya kamuwa da pylori ta hanyar cin abinci ko ruwa wanda ya gurɓace da al'amarin najasa.An nuna cewa maganin rigakafi a hade tare da mahadi na bismuth yana da tasiri wajen magance cutar H. Pylori mai aiki..H.A halin yanzu ana gano kamuwa da cutar pylori ta hanyoyin gwaji masu ɓarna bisa ga endoscopy da biopsy (watau histology, al'ada) ko hanyoyin gwaji marasa ɓarna kamar gwajin numfashi na urea (UBT), gwajin rigakafin serologic da gwajin antigen stool.UBT yana buƙatar kayan aikin lab masu tsada da amfani da reagent na rediyo.Gwaje-gwajen serologic antibody ba su bambanta tsakanin cututtukan da ke aiki a halin yanzu da abubuwan da suka gabata ko cututtukan da aka warke ba.Gwajin antigen stool yana gano antigen da ke cikin feces, wanda ke nuna kamuwa da cutar H. pylori mai aiki.Hakanan za'a iya amfani da shi don lura da tasirin magani da sake dawowar kamuwa da cuta. H. pylori Ag Rapid Test yana amfani da colloidal zinariya conjugated monoclonal anti-H.pylori antibody da wani monoclonal anti-H.pylori antibody don gano musamman H. pylori antigen da ke cikin samfurin mara lafiyar mara lafiya.Gwajin ya dace da mai amfani, daidai, kuma ana samun sakamakon a cikin mintuna 15.
KA'IDA
Gwajin H. pylori Ag Rapid Gwajin gwaji ne na sanwici a gefe yana gudana chromatographic immunoassay.Tarin gwajin ya ƙunshi: 1) wani kushin conjugate mai launin burgundy mai ɗauke da monoclonal anti-H.pylori antibody conjugated da colloidal zinariya (anti-Hp conjugates) da kuma 2) wani nitrocellulose membrane tsiri dauke da gwajin line (T line) da kuma sarrafawa line (C line).An riga an riga an lulluɓe layin T tare da wani anti-H na monoclonal.pylori antibody, kuma layin C an riga an yi masa rufi da goat anti-mouse IgG antibody.
Lokacin da isassun ƙarar samfur ɗin da aka fitar a cikin rijiyar kaset ɗin gwajin, samfurin yana ƙaura ta hanyar aikin capillary a cikin kaset ɗin.H. pylori antigens, idan akwai a cikin samfurin, za su ɗaure da anti-Hp conjugates. An kama immunocomplex a kan membrane ta hanyar riga-kafi mai rufi wanda ya samar da layin T mai launin burgundy, yana nuna sakamakon gwajin H. pylori mai kyau.Rashin layin T yana nuna cewa ƙaddamar da antigens na H. pylori a cikin samfurin yana ƙasa da matakin da ake iya ganowa, yana nuna sakamakon gwajin H. pylori. Gwajin ya ƙunshi kulawar ciki (layin C) wanda ya kamata ya nuna layin launi na burgundy. da immunocomplex na goat anti-mouse IgG / linzamin kwamfuta IgG-gold conjugate ko da kuwa da launi ci gaban a kan T line.Idan layin C bai haɓaka ba, sakamakon gwajin ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.
