TAKAITACCE DA BAYANIN GWAJI
Kwalara cuta ce mai saurin kamuwa da cuta wacce ke da yawan asarar ruwan jiki da na lantarki ta hanyar gudawa mai tsanani.An gano magungunan cutar kwalara a matsayin Vibrio cholerea (V. Cholerae), kwayar cutar gram negative, wacce gaba daya ke yadawa ga mutane ta gurbataccen ruwa da abinci.
An raba nau'in V. Cholerae zuwa ƙungiyoyin serogroups da yawa bisa tushen O antigens.Ƙungiyoyin O1 da O139 suna da sha'awa ta musamman domin dukansu na iya haifar da annoba da kwalara.Yana da mahimmanci a tantance da sauri kasancewar V. cholerae O1 da O139 a cikin samfuran asibiti, ruwa, da abinci ta yadda hukumomin kiwon lafiyar jama'a za su iya daukar matakan kariya da suka dace.
Za a iya amfani da gwajin gaggawa na Cholera Ag a cikin filin kai tsaye ta wurin ƙwararrun ma'aikata marasa horo ko ƙarancin ƙwarewa kuma ana samun sakamakon a ƙasa da mintoci 10, ba tare da ƙaƙƙarfan kayan aikin dakin gwaje-gwaje ba.
KA'IDA
Gwajin Cholera Ag Rapid Gwajin gwajin gwaji ne na chromatographic na gefe.Kaset ɗin gwajin ya ƙunshi: 1) wani pad mai launi mai launin burgundy mai ɗauke da monoclonal anti-V.Kwalara O1 da O139 antibodies conjugated da colloid zinariya (O1/O139-antibody conjugates) da zomo IgG-zinariya conjugates, 2) wani nitrocellulose membrane tsiri dauke da biyu gwajin band (1 da 139 makada) da kuma iko band (C band).An riga an riga an rufe rukunin 1 tare da monoclonal anti-V.Kwalara O1 antibody.Ƙungiyar 139 an riga an riga an rufe shi da monoclonal anti-V.Kwalara O139 antibody.An riga an riga an lulluɓe ƙungiyar C tare da rigakafin goat anti-mouse IgG antibody.
Lokacin da aka yi amfani da isasshen adadin samfurin gwaji a cikin rijiyar samfurin kaset ɗin gwajin, samfurin yana ƙaura ta hanyar aikin capillary a cikin kaset ɗin.V. Cholera O1/O139 antigen idan akwai a cikin samfurin zai ɗaure da daidaitaccen O1/O139-antibody zinariya conjugate.Ana kama wannan immunocomplex akan membrane ta anti-V da aka riga aka rufe.Cholera O1/O139 antibody, samar da bandeji mai launin burgundy, yana nuna kyakkyawan sakamakon gwajin Cholera O1/O139.Rashin ƙungiyar gwajin yana nuna sakamako mara kyau.
Gwajin yana ƙunshe da iko na ciki (C band) wanda yakamata ya nuna ƙungiyar launin burgundy na immunocomplex na goat anti-mouse IgG/ linzamin kwamfuta IgG-gold conjugate ba tare da la'akari da ci gaban launi akan rukunin gwajin ba.In ba haka ba, sakamakon gwajin ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.
