TAKAITACCE DA BAYANIN GWAJI
Chikungunya cuta ce mai saurin kamuwa da cuta ta hanyar cizon sauro mai cutar Aedes aegypti.Yana da kurji, zazzabi, da ciwon haɗin gwiwa mai tsanani (arthralgias) wanda yawanci yakan wuce kwanaki uku zuwa bakwai.Sunan ya samo asali ne daga kalmar Makonde mai ma'ana "abin da ya tanƙwara" dangane da yanayin da ya ɓullo da shi sakamakon alamun cututtukan arthritic na cutar.Yana faruwa ne a lokacin damina
a wurare masu zafi na duniya, musamman a Afirka, Kudu maso Gabashin Asiya, kudancin Indiya da Pakistan.Alamun yawanci yawanci nau'i ne na asibiti wanda ba a iya bambancewa waɗanda aka gani a zazzabin dengue.Lallai, an sami rahoton kamuwa da cuta guda biyu na dengue da chikungunya a Indiya.Ba kamar dengue ba, bayyanar cututtukan jini ba su da yawa kuma galibi cutar cuta ce mai iyakance kanta.Don haka yana da matukar muhimmanci
a asibiti bambance dengue daga CHIK kamuwa da cuta.An gano cutar CHIK bisa binciken serological da keɓewar ƙwayar cuta a cikin beraye ko al'adun nama.IgM immunoassay ita ce hanya mafi amfani da gwajin gwaji.Gwajin gaggawa na Chikungunya IgG/IgM yana amfani da antigens na sake haduwa da aka samo daga sunadarin tsarin sa, yana gano IgG/IgM anti-CHIK a cikin jini ko jini cikin jini cikin mintuna 20.Za a iya yin gwajin ta marasa horo ko
ƙwararrun ma'aikata kaɗan, ba tare da ƙaƙƙarfan kayan aikin dakin gwaje-gwaje ba.
KA'IDA
Gwajin gaggawa na Chikungunya IgG/IgM+NS1 antigen shine gwajin rigakafi na chromatographic na gefe.Kaset ɗin gwajin ya ƙunshi IgG/IgM Strip da NS1 tsiri.
IgG/IgM tsiri: 1) wani burgundy launi conjugate pad dauke da Chikungunya recombinant envelope antigens conjugated tare da colloid zinariya (dengue conjugates) da zomo IgG-gold conjugates,2) wani nitrocellulose membrane tsiri dauke da gwaji guda biyu makada (G da M bands) da kuma bandeji (C band).An riga an yi wa rukunin G band ɗin rigar riga-kafi don gano ƙwayar cuta ta IgG anti-Chikungunya, M band ɗin kuma an lulluɓe shi da maganin rigakafin IgM anti-Chikungunya virus, sannan an riga an yi wa rukunin C ɗin riga-kafi da riga-kafin akuya IgG.
NS1 tsiri:1) wani burgundy launi conjugate pad dauke da linzamin kwamfuta anti-Chikungunya NS1 antigen conjugated da colloid zinariya (Chikungunya Ab conjugates), 2) wani nitrocellulose membrane tsiri dauke da gwajin band (T band) da kuma kula da band (C band).An riga an lulluɓe ƙungiyar T tare da antigen anti-Chikungunya NS1 zomo, kuma rukunin C an riga an yi masa rufi da riga-kafi na rigakafin linzamin kwamfuta na IgG.
IgG/IgM tsiri: Lokacin da aka ba da isasshen adadin samfurin gwaji a cikin rijiyar samfurin kaset ɗin, samfurin yana ƙaura ta hanyar aikin capillary a cikin kaset ɗin.IgG anti-Chikungunya kwayar cutar idan akwai a cikin samfurin za ta daure da Chikungunya conjugates.Sa'an nan kuma reagent ɗin da aka lulluɓe a kan band ɗin G ya kama immunocomplex, yana samar da band mai launin burgundy, yana nuna sakamakon gwajin IgG mai kyau na Chikungunya kuma yana ba da shawarar kamuwa da cuta kwanan nan ko maimaitawa.IgM anti-Chikungunya kwayar cutar, idan akwai a cikin samfurin, za ta daure da Chikungunya conjugates.Sa'an nan aka kama immunocomplex ta reagent pre-rubutu a kan M band, kafa wani burgundy launi M band, nuna wani Chikungunya cutar IgM tabbatacce sakamakon gwajin da kuma bayar da shawarar wani sabon kamuwa da cuta.Rashin kowane nau'in gwaji (G da M) yana nuna sakamako mara kyau.Gwajin ya ƙunshi iko na ciki (C band) wanda yakamata ya nuna band mai launin burgundy na immunocomplex na goat anti rabbit IgG/
zomo IgG-gold conjugate ba tare da la'akari da ci gaban launi akan kowane rukunin T ba.In ba haka ba, sakamakon gwajin ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.
NS1 Strip: Lokacin da aka ba da isasshen adadin samfurin gwaji a cikin rijiyar samfurin kaset, samfurin yana ƙaura ta hanyar aikin capillary a cikin kaset ɗin gwajin.Chikungunya NS1 Ag idan akwai a cikin samfurin zai ɗaure ga haɗin gwiwar Chikungunya Ab.Ana kama immunocomplex akan membrane ta riga-kafin anti-NS1 zomo wanda aka rigaya, yana samar da rukunin T mai launin burgundy, yana nuna kyakkyawan sakamakon gwajin Chikungunya Ag.Rashin rukunin T yana nuna sakamako mara kyau.Gwajin ya ƙunshi iko na ciki (C band) wanda ya kamata ya nuna band mai launin burgundy na immunocomplex na goat anti-mouse IgG/ linzamin kwamfuta IgG-gold conjugate ko da kuwa kasancewar T band mai launi.In ba haka ba, sakamakon gwajin ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.
